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KMID : 0380020050200020100
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 2 p.100 ~ p.105
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Design of Denitrification Reactor by Using Permeabilized and Immobilized Paracoccus denitrificans
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Yoon Mi-Sun
Song Ju-Yeong
Park Geun-Ho
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Abstract
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Removal of nitrogen compound from waste water is essential and often accomplished by biological process. Denitrification bacterium, Paracoccus denitrificans (KCTC 2350) is employed to estimate the denitrification ability and the characteristics. In the immobilized biological reactor system, the measurement of absolute amount of active strain in the reactor is comparatively difficult or impossible. In this. study, a reactor was designed with the unwoven texture wrapped peep holed plastic tube to calculate the absolute amount of active strain by comparing the activity of the permeabilized and or immobilized reactor and the free cell reactor The reactor system was continuous stirred tank reactor and the reaction rate of substrate consumption was assumed to satisfy the Michaelis-Menten equation. The effluent concentration of nitrate and nitrite was measured to estimate the apparent parameter of Michaelis-Menten equation. As a result, we found that the amount of immobilized active strain was figured out to be half of the total active strain in the reactor and the time required to be reached in the equilibrium state in the permeabilized and or immobilized reactor system was figured out to be shorter than that of the free cell reactor system.
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KEYWORD
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Paracoccus denitrificans, CSTR, permeabilization, denitrification, immobilization, Michaelis-Menten
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